In this study we performed a stepāwise optimization of biologically active ILā2 for delivery using. E. coli Nissle 1917. Engineering of the strain was coupled with an in vitro cell assay to
probiotics may ļ¬t the deļ¬nition of LBPs and can be developed as Escherichia coli Nissle 1917 (EcN) has been used as a probiotic since its isolation over 100 years ago21. In its
Despite the great progress of current bacterially based biotherapeutics, their unsatisfying efficacy and underlying safety problems have limited their clinical application. Herein, inspired by probiotic Escherichia coli strain Nissle 1917, probiotic-derived outer membrane vesicles (OMVs) are found to serve as an effective therapeutic platform for the treatment of inflammatory bowel disease
We selected E. coli Nissle 1917 (EcN) as the chassis organism beĀ cause of its long history of safe use in human populations and the availability of numerous tools for genetic manipulation in this speĀ cies (39). EcN is widely used in Europe as a probiotic under the brand name Mutaflor. The excretion profile of orally dosed EcN was reĀ
We engineered probiotic E. coli Nissle 1917, to express and secrete the antimicrobial peptide, Microcin J25. Using in vitro experiments and an animal model of 300 turkeys, we establish the
Strain CFT073 is a bona fide uropathogen, whereas strains 83972 and Nissle 1917 are harmless probiotic strains of urinary tract and faecal origin, respectively. Despite their different environmental origins and dispositions the three strains are very closely related and the ancestors of 83972 and Nissle 1917 must have been very similar to CFT073.
AIM: To evaluate the effect of oral Escherichia coli (E. coli) Nissle application on the outcome of intestinal-borne dermatoses.. METHODS: In a randomized, controlled, non-blinded prospective clinical trial 82 patients with intestinal-borne facial dermatoses characterized by an erythematous papular-pustular rash were screened.