Kim J.K., Shin E.C., Park H.G. Fructooligosaccharides decreased the ability of probiotic Escherichia coli Nissle 1917 to adhere to co-cultures of human intestinal cell lines. J Korean Soc Appl Biol Chem. 2015; 58:45–52. doi: 10.1007/s13765-015-0002-5. [Google Scholar] Although the probiotic Escherichia coli strain Nissle 1917 has been proven to be efficacious for the treatment of inflammatory bowel diseases, the underlying mechanisms of action still remain elusive. The aim of the present study was to analyze the effects of E. coli Nissle 1917 on cell cycling and …
Escherichia coli Nissle 1917 Active UC: 5 Ɨ 10 10 bacteria twice daily until remission (maximum of 12 wk), followed by 5 Ɨ 10 10 bacteria daily for a maximum of 12 mo39; inactive UC: 5 Ɨ 10 10
ABSTRACT. Escherichia coli strain Nissle has been used as a probiotic and therapeutic agent for over a century. Reports suggest that Nissle protects mice from enterohemorrhagic E. coli (EHEC) O157:H7 strains; however, mice are not very susceptible to O157:H7 and are not accurate models for O157:H7 infection in humans.
In this study we performed a step‐wise optimization of biologically active IL‐2 for delivery using. E. coli Nissle 1917. Engineering of the strain was coupled with an in vitro cell assay to
probiotics may fit the definition of LBPs and can be developed as Escherichia coli Nissle 1917 (EcN) has been used as a probiotic since its isolation over 100 years ago21. In its Despite the great progress of current bacterially based biotherapeutics, their unsatisfying efficacy and underlying safety problems have limited their clinical application. Herein, inspired by probiotic Escherichia coli strain Nissle 1917, probiotic-derived outer membrane vesicles (OMVs) are found to serve as an effective therapeutic platform for the treatment of inflammatory bowel disease We selected E. coli Nissle 1917 (EcN) as the chassis organism be­ cause of its long history of safe use in human populations and the availability of numerous tools for genetic manipulation in this spe­ cies (39). EcN is widely used in Europe as a probiotic under the brand name Mutaflor. The excretion profile of orally dosed EcN was re­ We engineered probiotic E. coli Nissle 1917, to express and secrete the antimicrobial peptide, Microcin J25. Using in vitro experiments and an animal model of 300 turkeys, we establish the Strain CFT073 is a bona fide uropathogen, whereas strains 83972 and Nissle 1917 are harmless probiotic strains of urinary tract and faecal origin, respectively. Despite their different environmental origins and dispositions the three strains are very closely related and the ancestors of 83972 and Nissle 1917 must have been very similar to CFT073.
AIM: To evaluate the effect of oral Escherichia coli (E. coli) Nissle application on the outcome of intestinal-borne dermatoses.. METHODS: In a randomized, controlled, non-blinded prospective clinical trial 82 patients with intestinal-borne facial dermatoses characterized by an erythematous papular-pustular rash were screened.
Method: Genetically modified probiotic Escherichia coli Nissle 1917 (EcN-20) producing a potent water soluble antioxidant pyrroloquinoline quinone (PQQ) was supplemented with oral citric acid and compared with another genetically modified probiotic EcN-21 producing PQQ and citric acid against oxidative stress induced by Cd and Hg. Rats were
Inspired by the fact that probiotics colonized and grew in the mucus layer under physiological conditions, we developed a strategy for a super probiotic (EcN@TA-Ca 2+ @Mucin) coated with tannic acid and mucin via layer-by-layer technology. We demonstrated that mucin endows probiotics with superior resistance to the harsh environment of the CCK-8 assay, Hoechst 33258 staining and flow cytometry analysis were used for examining cytotoxicity of tumoricidal protein azurin toward CT26 cells. An engineered probiotic Ep-AH harboring azurin and hlpA genes was developed using Escherichia coli Nissle 1917 (EcN) chassis. 2hc3eA8.
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